Module Functional Analysis

The OmicsBox Functional Analysis module is a well-known comprehensive bioinformatics tool for functional annotation.

• High-Throughput Blast and InterProScan: Utilize CloudBlast and CloudInterProScan for rapid sequence alignments and domain searches against customizable reference datasets.
• Gene Ontology Mapping: Link potential homologs and domains with available functional annotation from the latest well-curated databases provided by UniProt and Gene Ontology consortia.
• Blast2GO Annotation: The Blast2GO methodology allows to flexibly assign the most reliable functional labels to novel sequence datasets, taking into account source annotation quality and ontology hierarchies.
• Enrichment Analysis: Use different enrichment analysis approaches (Fisher Exact Test and GSEA) to identify over and under-represented molecular functions.
• Combined Pathway Analysis: Identify Reactome and KEGG pathways for any set of sequences, utilize the differential expression data to compute pathway enrichment using (GSEA), and benefit from a combined visualization for easier insights.

Additional Resources

• Functional Annotation Analysis use case: https://www.biobam.com/whole-genome-functional-annotation-of-solanum-lycopersicum/ .

Functional Analysis Example Dataset: https://resources.biobam.com/omicsbox/example_data/version_2_0_0/FunctionalAnalysis.zip